Nocardioides nitrophenolicus sp. nov., a p-nitrophenol-degrading bacterium

Int J Syst Bacteriol. 1999 Apr;49 Pt 2:675-80. doi: 10.1099/00207713-49-2-675.

Abstract

A p-nitrophenol-degrading bacterial strain was isolated from industrial wastewater. This strain (NSP41T) was identified as a member of the genus Nocardioides from chemotaxonomic characterizations and phylogenetic inference based on 16S rDNA sequence analysis. The G + C content is 71.4 mol%. The diamino acid in the cell-wall peptidoglycan is LL-diaminopimelic acid. The predominant menaquinone is MK-8(H4). The cellular fatty acid profile is similar to those of Nocardioides species. 16S rDNA sequence analysis show that strain NSP41T is the most related to Nocardioides simplex strains with a level of nucleotide similarity of 98.6%. The levels of 16S rDNA similarity between strain NSP41T and other Nocardioides species ranged from 93.8 to 95.1%. This organism is distinguishable from some other Nocardioides species as well as N. simplex strains by DNA-DNA relatedness data. This organism is different from N. simplex strains in some phenotypic characteristics. Therefore, on the basis of the data presented, a new species of the genus Nocardioides, Nocardioides nitrophenolicus, is proposed. The type strain of the new species is strain NSP41T (= KCTC 0457BPT).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actinomycetales / classification*
  • Actinomycetales / cytology
  • Actinomycetales / genetics
  • Actinomycetales / physiology
  • Base Composition
  • Biodegradation, Environmental
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Fatty Acids / analysis
  • Genes, rRNA
  • Industrial Waste
  • Molecular Sequence Data
  • Nitrophenols / metabolism*
  • Phylogeny
  • RNA, Ribosomal / genetics
  • Sequence Analysis, DNA
  • Water Microbiology

Substances

  • DNA, Bacterial
  • DNA, Ribosomal
  • Fatty Acids
  • Industrial Waste
  • Nitrophenols
  • RNA, Ribosomal
  • 4-nitrophenol

Associated data

  • GENBANK/AF005024