Cloning, tissue expression, and chromosomal assignment of human MRJ gene for a member of the DNAJ protein family

J Hum Genet. 1999;44(3):185-9. doi: 10.1007/s100380050139.

Abstract

The DnaJ protein family consists of proteins with a highly conserved amino acid stretch called the "J-domain". A cDNA clone encoding a new protein with a J-domain was isolated from a human fetal brain cDNA library. This new member of the DnaJ family of 241 amino acid residues showed 94% identity with mouse Mrj (accession number, AF035962) and 71% identity with mouse Msj-1 (accession number, U95607) along its entire sequence. Reverse transcription-coupled polymerase chain reaction (RT-PCR) analysis showed the messenger RNA was ubiquitously expressed in various human tissues. The chromosomal location of the gene was determined by PCR-based analyses with both a human/rodent monochromosomal hybrid cell panel and a radiation hybrid panel to map on chromosome 11q25 region.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Chromosome Mapping
  • Chromosomes, Human, Pair 11
  • Cloning, Molecular
  • Conserved Sequence
  • DNA, Complementary / genetics
  • Genetic Markers
  • HSP40 Heat-Shock Proteins
  • Heat-Shock Proteins / genetics*
  • Humans
  • Hybrid Cells
  • Molecular Chaperones / genetics*
  • Molecular Sequence Data
  • Multigene Family*
  • Nerve Tissue Proteins*
  • RNA, Messenger / isolation & purification
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Homology, Amino Acid
  • Tissue Distribution

Substances

  • DNA, Complementary
  • DNAJB6 protein, human
  • Genetic Markers
  • HSP40 Heat-Shock Proteins
  • Heat-Shock Proteins
  • Molecular Chaperones
  • Nerve Tissue Proteins
  • RNA, Messenger

Associated data

  • GENBANK/AB014888