Comparisons among pimonidazole binding, oxygen electrode measurements, and radiation response in C3H mouse tumors

Radiat Res. 1999 May;151(5):580-9.


Pimonidazole binding was compared with oxygen electrode measurements and with measurements of the radiobiologically hypoxic fraction in C3H mammary tumors in which oxygenation was manipulated by means of subjecting tumor-bearing CDF1 mice to air breathing, carbogen breathing, oxygen breathing, hydralazine injection or tumor clamping. Hypoxia measured by pimonidazole binding could be correlated with both pO2 (r2 = 0.81) and radiobiologically hypoxic fraction (r2 = 0.85) in this system. The scope and limitation of pimonidazole as an immunohistochemical marker for tumor hypoxia is discussed.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Biomarkers
  • Cell Hypoxia*
  • Electrodes
  • Female
  • Immunohistochemistry
  • Male
  • Mammary Neoplasms, Experimental / blood supply
  • Mammary Neoplasms, Experimental / metabolism
  • Mammary Neoplasms, Experimental / radiotherapy*
  • Mice
  • Mice, Inbred C3H
  • Mice, Inbred DBA
  • Nitroimidazoles / metabolism*
  • Oxygen / analysis*
  • Radiation-Sensitizing Agents / metabolism*


  • Biomarkers
  • Nitroimidazoles
  • Radiation-Sensitizing Agents
  • pimonidazole
  • Oxygen