RESPONSIVE-TO-ANTAGONIST1, a Menkes/Wilson disease-related copper transporter, is required for ethylene signaling in Arabidopsis

Cell. 1999 Apr 30;97(3):383-93. doi: 10.1016/s0092-8674(00)80747-3.

Abstract

Ethylene is an important regulator of plant growth. We identified an Arabidopsis mutant, responsive-to-antagonist1 (ran1), that shows ethylene phenotypes in response to treatment with trans-cyclooctene, a potent receptor antagonist. Genetic epistasis studies revealed an early requirement for RAN1 in the ethylene pathway. RAN1 was cloned and found to encode a protein with similarity to copper-transporting P-type ATPases, including the human Menkes/Wilson proteins and yeast Ccc2p. Expression of RAN1 complemented the defects of a ccc2delta mutant, demonstrating its function as a copper transporter. Transgenic CaMV 35S::RAN1 plants showed constitutive expression of ethylene responses, due to cosuppression of RAN1. These results provide an in planta demonstration that ethylene signaling requires copper and reveal that RAN1 acts by delivering copper to create functional hormone receptors.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alkenes / pharmacology
  • Arabidopsis / enzymology
  • Arabidopsis / genetics*
  • Arabidopsis Proteins*
  • Biological Transport / genetics
  • Cation Transport Proteins*
  • Cloning, Molecular
  • Copper / metabolism*
  • Copper / pharmacokinetics
  • Copper Transport Proteins
  • Ethylenes / metabolism
  • Gene Expression / drug effects
  • Gene Expression / genetics
  • Gene Expression Regulation, Plant / drug effects
  • Genetic Testing
  • Hepatolenticular Degeneration / genetics*
  • Hepatolenticular Degeneration / metabolism
  • Humans
  • Molecular Sequence Data
  • Mutation / physiology
  • Phenotype
  • Plant Development
  • Plant Proteins / antagonists & inhibitors
  • Plant Proteins / genetics
  • Plant Proteins / metabolism
  • Plants / metabolism
  • Plants, Genetically Modified
  • Protein Kinases / genetics*
  • Protein Kinases / metabolism
  • Receptors, Cell Surface / antagonists & inhibitors
  • Receptors, Cell Surface / genetics
  • Receptors, Cell Surface / metabolism
  • Sequence Homology, Amino Acid
  • Signal Transduction / genetics*
  • Yeasts / enzymology
  • Yeasts / genetics

Substances

  • Alkenes
  • Arabidopsis Proteins
  • Cation Transport Proteins
  • Copper Transport Proteins
  • Ethylenes
  • Plant Proteins
  • RAN1 protein, Arabidopsis
  • Receptors, Cell Surface
  • ethylene receptors, plant
  • Copper
  • ethylene
  • Protein Kinases

Associated data

  • GENBANK/AF082565
  • GENBANK/AF091112