Sonochemically induced radicals generated by pulsed high-energy ultrasound in vitro and in vivo

Ultrasound Med Biol. 1999 Feb;25(2):301-6. doi: 10.1016/s0301-5629(98)00143-4.


The aim of this study was to evaluate the role of radicals as a mechanism of tissue damage induced by pulsed high-energy ultrasound. Transient cavitation has proved to be an important mechanism for the generation of reactive radical species during pulsed high-energy ultrasound applications. The amount of radicals studied in in vitro experiments using a chemical dosimeter based on iodine release is proportional to the number of pulses. Sonications of the R3327-AT1 subline of the Dunning prostate rat tumor transplanted in the thigh of Copenhagen rats were performed applying 500 and 2000 pulses at a pulse repetition frequency of 1 Hz. Tumor growth after treatment was compared with sham-treated controls. We were able to assess a significant growth delay, but could not find a significant difference between the two groups treated. In conclusion, radical formation does not seem to be the major mechanism for tissue necrosis induced by pulsed high-energy ultrasound.

MeSH terms

  • Adenocarcinoma / metabolism
  • Adenocarcinoma / therapy*
  • Animals
  • Free Radicals
  • Lithotripsy
  • Male
  • Neoplasm Transplantation
  • Prostatic Neoplasms / metabolism
  • Prostatic Neoplasms / therapy*
  • Rats
  • Ultrasonic Therapy*


  • Free Radicals