Evidence for oestrogen receptor alpha-immunoreactivity in gonadotrophin-releasing hormone-expressing neurones

J Neuroendocrinol. 1999 May;11(5):331-5. doi: 10.1046/j.1365-2826.1999.00347.x.

Abstract

Having used the cingulate cortex to demonstrate the validity of our methods for detecting hitherto unrecognized oestrogen receptor alpha (ERalpha)-immunoreactive neurones, we have now employed immunoprecipitation and double-label immunohistochemistry to investigate whether the ERalpha protein is present in gonadotrophin-releasing hormone (GnRH)-containing cells. The immortalized GnRH cell line GT1-7 and GnRH neurones within the rat preoptic area were found to possess ERalpha-immunoreactivity (ERalpha-IR). These observations indicate that oestrogen may regulate the synthesis and release of GnRH by direct actions on GnRH neurones.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acrolein
  • Animals
  • Antibody Specificity
  • Artifacts
  • Cell Extracts
  • Cell Line
  • Cell Nucleus / chemistry
  • Estrogen Receptor alpha
  • Female
  • Formaldehyde
  • Glutaral
  • Gonadotropin-Releasing Hormone / metabolism*
  • Immunohistochemistry
  • Molecular Weight
  • Neurons / chemistry*
  • Neurons / cytology
  • Neurons / metabolism
  • Ovariectomy
  • Polymers
  • Precipitin Tests
  • Preoptic Area / cytology*
  • Rats
  • Receptors, Estrogen / analysis*
  • Receptors, Estrogen / immunology
  • Reproducibility of Results

Substances

  • Cell Extracts
  • Estrogen Receptor alpha
  • Polymers
  • Receptors, Estrogen
  • Formaldehyde
  • Gonadotropin-Releasing Hormone
  • Acrolein
  • Glutaral
  • paraform