Nuclear translocation of GAPDH-GFP fusion protein during apoptosis

Neuroreport. 1999 Apr 6;10(5):1149-53. doi: 10.1097/00001756-199904060-00045.


Increased expression and nuclear accumulation of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) are early, critical events in several forms of apoptosis. In order to investigate the subcellular trafficking of GAPDH in vivo, the localization of a GAPDH-green fluorescent protein (GFP) fusion was studied in PC12, HEK 293 and COS-1 cells. In control cells, fusion protein autofluorescence was largely restricted to the cytoplasm, rather than the nuclear concentration favored by GFP alone. In contrast, as early as 30 min after an insult, nuclear fluorescence increased in all cell lines studied. The fusion protein redistribution paralleled the dynamics of endogenous GAPDH. These data suggest that some nuclear GAPDH observed during apoptosis represents protein previously resident in the cytosol. This construct provides an in vivo monitor for an early change in apoptosis.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Apoptosis / physiology*
  • Biological Transport / physiology
  • COS Cells
  • Cell Nucleus / metabolism*
  • Glyceraldehyde-3-Phosphate Dehydrogenases / genetics
  • Glyceraldehyde-3-Phosphate Dehydrogenases / metabolism
  • Green Fluorescent Proteins
  • Humans
  • Indicators and Reagents / metabolism*
  • Luminescent Proteins / genetics
  • Luminescent Proteins / metabolism*
  • PC12 Cells
  • Rats
  • Recombinant Fusion Proteins / metabolism*
  • Subcellular Fractions / metabolism
  • Time Factors
  • Tissue Distribution / physiology


  • Indicators and Reagents
  • Luminescent Proteins
  • Recombinant Fusion Proteins
  • Green Fluorescent Proteins
  • Glyceraldehyde-3-Phosphate Dehydrogenases