Effect of ion modification of commonly used orthopedic materials on the attachment of human bone-derived cells

J Biomed Mater Res. 1999 Jun 15;45(4):345-54. doi: 10.1002/(sici)1097-4636(19990615)45:4<345::aid-jbm9>3.0.co;2-j.

Abstract

Biomaterials which combine optimum properties of strength and biocompatibility are desirable in improving the long-term performance of implantable medical devices. Our study is aimed at developing technology designed to alter the outer atomic layers of a material to give the desired compatibility with the tissue while retaining the properties of the bulk substratum. Materials used in this study were titanium vanadium alloy (Ti-6Al-4V) and cobalt chromium molybdenum alloy (Co-Cr). Soda lime glass discs and polyethylene terephthalate (PET) acted as controls. A cathode of either Ti-6Al-4V or Co-Cr was used to simultaneously deposit and implant identified substrata. The attachment of human bone-derived cells (HBDC) to various materials was determined using radiolabeling or colorimetric assays. Results show that HBDC adhere preferentially to the unmodified surfaces of Ti-6Al-4V and Ti-6Al-4V on glass compared to the unmodified Co-Cr surfaces and to that of the Co-Cr on glass. Depositing Ti-6Al-4V on Co-Cr gives significantly better attachment of HBDC than when depositing Co-Cr onto Ti-6Al-4V. While cellular attachment to the created surfaces reflects that of the cathodic materials, it is not identical to these materials. Ion deposition/implantation is capable of creating permanent surfaces which reflect the adhesion of source materials not bulk substrata.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alloys
  • Biocompatible Materials / chemistry*
  • Biocompatible Materials / pharmacology*
  • Bone and Bones / cytology*
  • Calcium Compounds / chemistry
  • Calcium Compounds / pharmacology
  • Cell Adhesion / drug effects
  • Cells, Cultured
  • Chromium Alloys / chemistry
  • Chromium Alloys / pharmacology
  • Colorimetry
  • Electron Probe Microanalysis
  • Glass
  • Humans
  • Materials Testing
  • Microscopy, Electron, Scanning
  • Oxides / chemistry
  • Oxides / pharmacology
  • Polyethylene Terephthalates / chemistry
  • Polyethylene Terephthalates / pharmacology
  • Sodium Hydroxide / chemistry
  • Sodium Hydroxide / pharmacology
  • Surface Properties
  • Tetrazolium Salts
  • Thiazoles
  • Titanium / chemistry
  • Titanium / pharmacology
  • X-Ray Diffraction

Substances

  • Alloys
  • Biocompatible Materials
  • Calcium Compounds
  • Chromium Alloys
  • Oxides
  • Polyethylene Terephthalates
  • Tetrazolium Salts
  • Thiazoles
  • titanium alloy (TiAl6V4)
  • soda lime
  • Sodium Hydroxide
  • Titanium
  • thiazolyl blue