Identification of T-DNA tagged Arabidopsis mutants that are resistant to transformation by Agrobacterium

Mol Gen Genet. 1999 Apr;261(3):429-38. doi: 10.1007/s004380050985.

Abstract

We have identified T-DNA tagged Arabidopsis mutants that are resistant to transformation by Agrobacterium tumefaciens (rat mutants). These mutants are highly recalcitrant to the induction of both crown gall tumors and phosphinothricin-resistant calli. The results of transient GUS (beta-glucuronidase) assays suggest that some of these mutants are blocked at an early step in the Agrobacterium-mediated transformation process, whereas others are blocked at a step subsequent to translocation of T-DNA into the nucleus. Attachment of Agrobacterium to roots of the mutants rat1 and rat3 was decreased under various incubation conditions. In most mutants, the transformation-deficient phenotype co-segregated with the kanamycin resistance encoded by the mutagenizing T-DNA. In crosses with susceptible wild-type plants, the resistance phenotype of many of these mutants segregated either as a semi-dominant or dominant trait.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Agrobacterium tumefaciens / genetics*
  • Agrobacterium tumefaciens / physiology
  • Arabidopsis / genetics*
  • Arabidopsis / microbiology*
  • Bacterial Adhesion
  • Crosses, Genetic
  • DNA, Bacterial / genetics*
  • Genes, Plant
  • Mutation*
  • Plant Roots / microbiology
  • Transformation, Genetic*

Substances

  • DNA, Bacterial
  • T-DNA