Capture of a genomic HMG domain sequence by the En/Spm-related transposable element Tpn1 in the Japanese morning glory

Mol Gen Genet. 1999 Apr;261(3):447-51. doi: 10.1007/s004380050987.

Abstract

The non-autonomous transposable element Tpn1 from the Japanese morning glory is an En/Spm-related DNA element found in the second intron of the DFR-B gene for flower pigmentation in the mutable line flecked, which shows variegation for flower color. It carries a genomic DNA segment containing at least four exon sequences encoding part of a HMG-box sequence. Spliced hybrid transcripts containing the DFR-B exon(s) and the HMG exons in Tpn1 were detected in the flower buds of the flecked line, and they were polyadenylated within Tpn1. Thus, Tpn1 can be regarded as a specialized transducing transposon carrying a part of the genomic sequence for a HMG box. The possible implications of the finding for evolution are discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • DNA Primers / genetics
  • DNA Transposable Elements / genetics*
  • DNA, Complementary / genetics
  • DNA, Plant / genetics
  • Genome, Plant
  • High Mobility Group Proteins / genetics*
  • Magnoliopsida / genetics*
  • Magnoliopsida / metabolism
  • Molecular Sequence Data
  • Mutation
  • Plant Proteins / genetics*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • RNA, Plant / genetics
  • RNA, Plant / metabolism
  • Sequence Homology, Amino Acid

Substances

  • DNA Primers
  • DNA Transposable Elements
  • DNA, Complementary
  • DNA, Plant
  • High Mobility Group Proteins
  • Plant Proteins
  • RNA, Messenger
  • RNA, Plant

Associated data

  • GENBANK/AB013348
  • GENBANK/AB013349
  • GENBANK/AB013350
  • GENBANK/AB013351
  • GENBANK/AB013352