Anti-P-selectin antibody attenuates rat brain ischemic injury

Neurosci Lett. 1999 Apr 23;265(3):163-6. doi: 10.1016/s0304-3940(99)00229-3.

Abstract

We examined a protective effect of anti-P-selectin monoclonal antibody against rat ischemic brain injury with 24 h of middle cerebral artery occlusion (MCAO). Anti-rat P-selectin monoclonal antibody, was intravenously injected at a dose of 1 mg/kg at 5 min before MCAO. Control animals received the same volume of vehicle. MCAO was accomplished by an insertion of nylon thread with silicone coating for 24 h. Application of anti-P-selectin antibody significantly reduced infarct size and brain water content at 24 h of MCAO. Although leukocyte infiltration was not normally detected, it became remarkably evident at 1 day of MCAO. However, treatment with ARP 2-4 significantly reduced the number of leukocytes. These results demonstrated that administration of monoclonal antibody against P-selectin attenuated infarct size and brain edema, which was associated with reduction of leukocyte infiltration.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / pharmacology*
  • Arterial Occlusive Diseases / immunology
  • Arterial Occlusive Diseases / therapy
  • Brain Chemistry
  • Brain Edema / immunology
  • Brain Edema / therapy
  • Brain Ischemia / immunology*
  • Brain Ischemia / therapy*
  • Cerebral Infarction / immunology
  • Cerebral Infarction / therapy
  • Disease Models, Animal
  • Immunohistochemistry
  • Lymphocyte Function-Associated Antigen-1 / analysis
  • Male
  • P-Selectin / immunology*
  • Rats
  • Rats, Wistar

Substances

  • Antibodies, Monoclonal
  • Lymphocyte Function-Associated Antigen-1
  • P-Selectin