Dictyostelium 17S, 25S, and 5S rDNAs lie within a 38,000 base pair repeated unit

Cell. 1976 Nov;9(3):431-8. doi: 10.1016/0092-8674(76)90088-x.

Abstract

Mapping with the restriction enzymes Sal 1 and R1 has generated a picture of the organization of Dictyostelium ribosomal DNA. The DNA which codes for 17S and 25S ribosomal RNAs is located within a stretch of repetitive DNA at least 38,000 base pairs long. This repeated unit includes 5S DNA, linked to 25S DNA. Two techniques were especially useful in the mapping: "cloning" 14S + 25S DNA on the plasmid pMB9 to amplify individual R1 fragments, and digesting DNA with R1 in the presence of the antibiotic distamycin A to produce specific partial digestion products.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Base Sequence
  • DNA / analysis*
  • DNA / metabolism
  • DNA Restriction Enzymes
  • DNA, Recombinant
  • Dictyostelium / analysis*
  • Distamycins
  • Myxomycetes / analysis*
  • Nucleic Acid Hybridization
  • Plasmids
  • RNA, Ribosomal / biosynthesis*

Substances

  • DNA, Recombinant
  • Distamycins
  • RNA, Ribosomal
  • DNA
  • DNA Restriction Enzymes