Removal of vegetal yolk causes dorsal deficencies and impairs dorsal-inducing ability of the yolk cell in zebrafish

Mech Dev. 1999 Mar;81(1-2):51-63. doi: 10.1016/s0925-4773(98)00202-0.

Abstract

To examine the nature of cytoplasm determinants for dorsal specification in zebrafish, we have developed a method in which we remove the vegetal yolk hemisphere of early fertilized eggs (vegetal removed embryos). When the vegetal yolk mass was removed at the 1-cell stage, the embryos frequently exhibited typical ventralized phenotypes: no axial structures developed. The frequency of dorsal defects decreased when the operation was performed at later stages. Furthermore, the yolk cell obtained from the vegetal-removed embryos lost the ability to induce goosecoid in normal blastomeres while the normal yolk cell frequently did so in normal and vegetal-removed embryos. These results suggested that the vegetal yolk cell mass contains the dorsal determinants, and that the dorsal-inducing ability of the yolk cell is dependent on the determinants.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blastomeres / physiology
  • Body Patterning
  • Fetal Proteins / metabolism
  • Gene Expression Regulation, Developmental
  • Goosecoid Protein
  • Homeodomain Proteins / metabolism
  • In Situ Hybridization
  • Models, Biological
  • Molecular Sequence Data
  • Repressor Proteins*
  • T-Box Domain Proteins*
  • Time Factors
  • Transcription Factors*
  • Yolk Sac / physiology*
  • Zebrafish / embryology*
  • Zebrafish Proteins*

Substances

  • Fetal Proteins
  • Goosecoid Protein
  • Homeodomain Proteins
  • Repressor Proteins
  • T-Box Domain Proteins
  • Transcription Factors
  • Zebrafish Proteins
  • gsc protein, zebrafish
  • Brachyury protein

Associated data

  • GENBANK/U63292