Dynamic expression and cellular localization of the drosophila 14-3-3epsilon during embryonic development

Mech Dev. 1999 Mar;81(1-2):209-12. doi: 10.1016/s0925-4773(98)00238-x.


The 14-3-3 protein family is known to interact with various proteins involved in signaling pathways. We report here the expression pattern of the Drosophila 14-3-3 (d14-3-3epsilon) protein during embryonic development. In syncytial blastoderm when the nuclei divided rapidly, d14-3-3epsilon localized in the nuclei. During cellularization d14-3-3epsilon gradually became membrane-bound. During gastrulation, an enhanced staining in the perinuclear region was observed in various tissues. Co-labeling with dp-ERK which recognized the activated form of MAPK suggested that d14-3-3epsilon was expressed prior to MAPK activation. During neuronal differentiation, the d14-3-3epsilon protein remained at a high level in the neuronal cytoplasm.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 14-3-3 Proteins
  • Animals
  • Blotting, Western
  • Drosophila / embryology*
  • Gastrula / metabolism
  • Gene Expression Regulation, Developmental
  • Neurons / metabolism
  • Proteins / analysis*
  • Proteins / metabolism*
  • Recombinant Fusion Proteins
  • Time Factors
  • Tissue Distribution
  • Tyrosine 3-Monooxygenase*


  • 14-3-3 Proteins
  • Proteins
  • Recombinant Fusion Proteins
  • Tyrosine 3-Monooxygenase