Comparison of a competitive inhibition ELISA and the card agglutination test for detection of antibodies to Anaplasma marginale and Anaplasma centrale in cattle

Aust Vet J. 1999 Apr;77(4):245-9. doi: 10.1111/j.1751-0813.1999.tb11712.x.

Abstract

Objective: To compare a recently developed recombinant MSP-5 competitive inhibition ELISA with a card agglutination test for detection of antibodies to Anaplasma marginale and Anaplasma centrale in Australian cattle.

Materials and methods: The ELISA was compared with the card agglutination test using 208 sera from cattle in Anaplasma-free herds, 86 sera from cattle experimentally infected with A marginale or A centrale and 757 sera from cattle in areas endemic for A marginale.

Results: The specificity of the ELISA, based on testing 208 sera from cattle in Anaplasma-free areas, was 99.5%, and the sensitivities for detection of antibodies to A marginale and A centrale in sera from the experimentally infected cattle were 98.0% and 100%, respectively. For the same sets of sera, the specificity of the card agglutination test was 98.6% and the sensitivities for detection of antibodies to A marginale and A centrale were 98.0% and 100%, respectively. For the 757 sera collected from cattle in areas endemic for A marginale, the agreement between the ELISA and the card agglutination test depended on the positive threshold selected for the ELISA. The maximum achievable agreement was 91.5% (kappa = 0.73; 95% confidence interval 0.66, 0.79).

Conclusion: We conclude that the competitive inhibition ELISA is a useful alternative to the card agglutination test for detection of A marginale or A centrale infection in cattle. The assay should be particularly useful for epidemiological applications such as prevalence studies and control programs.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Agglutination Tests / veterinary*
  • Anaplasma / immunology*
  • Anaplasma / isolation & purification
  • Anaplasmosis / blood
  • Anaplasmosis / diagnosis*
  • Anaplasmosis / prevention & control
  • Animals
  • Antibodies, Bacterial / blood
  • Antibodies, Bacterial / isolation & purification*
  • Cattle
  • Cattle Diseases / blood
  • Cattle Diseases / diagnosis*
  • Cattle Diseases / prevention & control
  • Enzyme-Linked Immunosorbent Assay / veterinary*
  • Sensitivity and Specificity

Substances

  • Antibodies, Bacterial