Structures and activities of cyclic ADP-ribose, NAADP and their metabolic enzymes

Mol Cell Biochem. 1999 Mar;193(1-2):89-98. doi: 10.1007/978-1-4419-8740-2_13.


ADP-ribosyl cyclase and CD38 are multi-functional enzymes involved in calcium signaling. Both can cyclize NAD and its guanine analog, NGD, at two different sites of the purine ring, N1 and N7, respectively, to produce cyclic ADP-ribose (cADPR) and cyclic GDP-ribose, a fluorescent but inactive analog. Both enzymes can also catalyze the exchange of the nicotinamide group of NADP with nicotinic acid, producing yet another potent activator of Ca2+ mobilization, nicotinic acid adenine dinucleotide phosphate (NAADP). The Ca2+ release mechanism activated by NAADP is totally independent of cADPR and inositol trisphosphate indicating it is a novel and hitherto unknown Ca2+ signaling pathway. This article summarizes the current results on the structures and activities of cADPR, NAADP and the enzymes that catalyze their syntheses. A comprehensive model accounting for the novel multi-functionality of ADP-ribosyl cyclase and CD38 is presented.

Publication types

  • Review

MeSH terms

  • ADP-ribosyl Cyclase
  • ADP-ribosyl Cyclase 1
  • Adenosine Diphosphate Ribose / analogs & derivatives
  • Adenosine Diphosphate Ribose / chemistry*
  • Adenosine Diphosphate Ribose / metabolism*
  • Antigens, CD*
  • Antigens, Differentiation / metabolism
  • Calcium / metabolism
  • Catalysis
  • Dose-Response Relationship, Drug
  • Models, Chemical
  • Models, Genetic
  • Models, Molecular
  • NAD+ Nucleosidase / metabolism
  • NADP / analogs & derivatives*
  • NADP / chemistry
  • NADP / metabolism
  • Sequence Homology, Amino Acid
  • Structure-Activity Relationship
  • Time Factors


  • Antigens, CD
  • Antigens, Differentiation
  • Adenosine Diphosphate Ribose
  • NADP
  • ADP-ribosyl Cyclase
  • NAD+ Nucleosidase
  • ADP-ribosyl Cyclase 1
  • Calcium