Genomic organization and chromosomal location of the mouse vasoactive intestinal polypeptide 1 (VPAC1) receptor

Genomics. 1999 May 15;58(1):90-3. doi: 10.1006/geno.1999.5805.

Abstract

The gene encoding the mouse vasoactive intestinal polypeptide type 1 (VPAC1) receptor was cloned, and its structural organization was determined. The gene (Vipr1) is more than 16 kb in length and is divided into 13 exons. The 5'-flanking region is highly GC-rich and lacks an apparent TATA box, but contains a CCAAT box, three potential Sp1-binding sites, and two potential AP-2-binding sites. Promoter analysis of the 5'-flanking region of Vipr1 using a luciferase gene reporter system revealed that the isolated 5'-flanking region has functional promoter activity. The mouse Vipr1 gene is encoded by a single gene, which was mapped to the distal region of mouse chromosome 9. This region is syntenic with human chromosome 3p, where the human VPAC1 receptor gene has been mapped.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Binding Sites
  • Chromosome Mapping
  • Chromosomes / genetics
  • Colforsin / pharmacology
  • DNA / chemistry
  • DNA / genetics
  • Exons
  • Gene Expression Regulation / drug effects
  • Genes / genetics*
  • Introns
  • Luciferases / genetics
  • Luciferases / metabolism
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred Strains
  • Molecular Sequence Data
  • Muridae
  • PC12 Cells
  • Promoter Regions, Genetic / genetics
  • Rats
  • Receptors, Vasoactive Intestinal Peptide / genetics*
  • Receptors, Vasoactive Intestinal Polypeptide, Type I
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Sequence Analysis, DNA
  • Tetradecanoylphorbol Acetate / pharmacology
  • Transcription, Genetic

Substances

  • Receptors, Vasoactive Intestinal Peptide
  • Receptors, Vasoactive Intestinal Polypeptide, Type I
  • Recombinant Fusion Proteins
  • Colforsin
  • DNA
  • Luciferases
  • Tetradecanoylphorbol Acetate

Associated data

  • GENBANK/AB022848
  • GENBANK/AB022849
  • GENBANK/AB022850
  • GENBANK/AB022851
  • GENBANK/AB022852
  • GENBANK/AB022853
  • GENBANK/AB022854
  • GENBANK/AB022855
  • GENBANK/AB022856
  • GENBANK/AB022857
  • GENBANK/AB022858
  • GENBANK/AB022859
  • GENBANK/AB022860