Dose-response relationships for hepatic aflatoxin B1-DNA adduct formation in the rat in vivo and in vitro: the use of immunoslot blotting for adduct quantitation

Teratog Carcinog Mutagen. 1999;19(2):157-70. doi: 10.1002/(sici)1520-6866(1999)19:2<157::aid-tcm8>;2-v.


An immunoslot blotting (ISB) method for quantitating aflatoxin B1-DNA adduct levels has been developed and used to examine the relationship between dose and hepatic aflatoxin B1-DNA adduct levels in rats fed aflatoxin B1 (AFB1) in the diet at dose levels of between 0.5 and 10 micrograms/kg/day. The method has also been used to examine the dose-response relationship for adduct formation in precision-cut rat liver slices incubated with AFB1 at concentrations between 0.01 and 2 microM. For the feeding studies, groups of male Fisher F344 rats were given AFB1 in the diet for periods of 1 to 10 weeks and hepatic DNA adduct levels determined using ISB. The time for adduct levels to reach steady-state conditions was determined in animals given approximately 10 micrograms of AFB1/kg/day and steady-state levels at lower concentrations measured. The time course for the accumulation of AFB1-DNA adducts in rat liver slices incubated with AFB1 at 0.5 microM has been investigated and the relationship between adduct formation and AFB1 concentration over a wide concentration range in liver slices has been determined.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aflatoxin B1 / pharmacology*
  • Animals
  • DNA Adducts / biosynthesis*
  • DNA Damage
  • Dose-Response Relationship, Drug*
  • Immunoblotting
  • In Vitro Techniques
  • Liver / drug effects*
  • Male
  • Rats
  • Rats, Inbred F344
  • Rats, Sprague-Dawley
  • Time Factors


  • DNA Adducts
  • Aflatoxin B1