Magnetic resonance imaging-based compartmentation and its application to measuring metabolite concentrations in the frontal lobe

Magn Reson Med. 1999 May;41(5):883-8. doi: 10.1002/(sici)1522-2594(199905)41:5<883::aid-mrm5>;2-b.


Partial volume mixing of water compartments within a spectroscopy voxel (e.g. cerebrospinal fluid within a "brain" voxel) may, if not corrected for, lead to underestimation of brain metabolite concentrations. To correct for this source of bias, a new imaging-based method of compartmentation analysis is presented. Brain water, cerebrospinal fluid and solid matter content were obtained from proton density- and T2-weighted images of the brain and an external standard in 10 healthy young males (21 to 30 years), and results compared with a previously-described technique based on spectroscopy. Mean (SD) fractional water content (betaMR) of the 2 x 2 x 2 cm3 voxel in the frontal lobes was 0.79 (0.03) by imaging, slightly but significantly (p = 0.03) smaller than the value of 0.83 (0.03) obtained by spectroscopy. From water-suppressed spectra recorded at five echo times, using betaMR determined by imaging, the T2-corrected concentrations of compounds containing N-acetylaspartate, creatine, choline and myo-inositol were 10.6 (1.0), 8.0 (0.9), 1.6 (0.3) and 3.7 (0.7) mmol.l(-1) of brain, respectively. Imaging-based compartmentation is a rapid and straightforward technique, and can be performed on standard MR systems.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Algorithms
  • Aspartic Acid / analogs & derivatives
  • Aspartic Acid / analysis
  • Body Water / chemistry
  • Cerebrospinal Fluid / chemistry
  • Choline / analysis
  • Creatine / analysis
  • Frontal Lobe / metabolism*
  • Humans
  • Image Processing, Computer-Assisted / methods
  • Inositol / analysis
  • Magnetic Resonance Imaging / methods*
  • Magnetic Resonance Spectroscopy / methods
  • Male
  • Signal Processing, Computer-Assisted


  • Aspartic Acid
  • Inositol
  • N-acetylaspartate
  • Creatine
  • Choline