Structure and regulation of OPR1 and OPR2, two closely related genes encoding 12-oxophytodienoic acid-10,11-reductases from Arabidopsis thaliana

Planta. 1999 Apr;208(2):155-65. doi: 10.1007/s004250050545.

Abstract

The genes of two closely related 12-oxophytodienoic acid reductases (EC 1.3.1.42), OPR1 and OPR2, were identified on a 7079-bp-long genomic fragment from Arabidopsis thaliana (L.) Heynh. The organization of these two genes was determined and putative cis elements were identified. Promoter-beta-glucuronidase (GUS) fusions expressed in transgenic Arabidopsis thaliana and Nicotiana tabacum L. plants revealed differences in OPR-promoter-driven GUS expression in flowers. While the OPR1 promoter directed GUS expression in young seeds, the OPR2 promoter directed pollen-specific expression. Both OPR1 and OPR2, were predominantly expressed in roots. Stress treatments, like local and systemic wounding, UV-C illumination and coldness, resulted in transient changes in steady-state OPR mRNA levels, but no concurrent changes in polypeptide level or enzyme activity were detected.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / enzymology*
  • Arabidopsis / genetics
  • Base Sequence
  • Cloning, Molecular
  • DNA, Plant
  • Gene Expression Regulation, Enzymologic*
  • Gene Expression Regulation, Plant*
  • Genes, Plant
  • Molecular Sequence Data
  • Oxidoreductases / genetics*
  • Oxidoreductases Acting on CH-CH Group Donors*
  • Plants, Genetically Modified
  • Plants, Toxic
  • Promoter Regions, Genetic
  • Tobacco

Substances

  • DNA, Plant
  • Oxidoreductases
  • Oxidoreductases Acting on CH-CH Group Donors
  • 12-oxophytodienoate reductase

Associated data

  • GENBANK/U92460