Expression of transforming growth factor-beta1, 2, 3 isoforms and type I and II receptors in acute focal cerebral ischemia: an immunohistochemical study in rat after transient and permanent occlusion of middle cerebral artery

Acta Neuropathol. 1999 May;97(5):447-55. doi: 10.1007/s004010051013.


Transforming growth factor beta (TGF-beta) is involved in the modulation of cell growth, differentiation and repair following injury of various organs. Previous studies on human autopsy material have indicated that TGF-beta isoforms-beta1, -beta2 and -beta3, and TGF-beta receptor type I are expressed in various cells of necrotizing brain lesions like infarction and abscess. The present immunohistochemical study was designed to investigate changes that may occur with regard to TGF-beta and its receptors type I and II in a rat model of focal brain ischemia induced by transient or permanent occlusion of the middle cerebral artery. Our findings indicate that at days 1 and 3 following such transient and permanent ischemia there is an up-regulation of TGF-beta isoforms -beta1, -beta2 and -beta3, and TGF-beta receptor types I and II mainly in the perifocal neurons, reactive astroglial cells, endothelial cells and macrophages.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Humans
  • Immunohistochemistry
  • Ischemic Attack, Transient / pathology*
  • Male
  • Middle Cerebral Artery / pathology*
  • Protein Isoforms*
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, Transforming Growth Factor beta / analysis*
  • Transforming Growth Factor beta / analysis*


  • Protein Isoforms
  • Receptors, Transforming Growth Factor beta
  • Transforming Growth Factor beta