A Cytosolic Catalase Is Needed to Extend Adult Lifespan in C. Elegans daf-C and clk-1 Mutants

Nature. 1999 May 13;399(6732):162-6. doi: 10.1038/20208.

Abstract

The dauer larva is an alternative larval stage in Caenorhabditis elegans which allows animals to survive through periods of low food availability. Well-fed worms live for about three weeks, but dauer larvae can live for at least two months without affecting post-dauer lifespan. Mutations in daf-2 and age-1, which produce a dauer constitutive (Daf-C) phenotype, and in clk-1, which are believed to slow metabolism, markedly increase adult lifespan. Here we show that a ctl-1 mutation reduces adult lifespan in otherwise wild-type animals and eliminates the daf-c and clk-1-mediated extension of adult lifespan. ctl-1 encodes an unusual cytosolic catalase; a second gene, ctl-2, encodes a peroxisomal catalase. ctl-1 messenger RNA is increased in dauer larvae and adults with the daf-c mutations. We suggest that the ctl-1 catalase is needed during periods of starvation, as in the dauer larva, and that its misexpression in daf-c and clk-1 adults extends lifespan. Cytosolic catalase may have evolved to protect nematodes from oxidative damage produced during prolonged dormancy before reproductive maturity, or it may represent a general mechanism for permitting organisms to cope with the metabolic changes that accompany starvation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.
  • Retracted Publication

MeSH terms

  • Animals
  • Caenorhabditis elegans / enzymology
  • Caenorhabditis elegans / genetics
  • Caenorhabditis elegans / physiology*
  • Caenorhabditis elegans Proteins*
  • Catalase / genetics
  • Catalase / physiology*
  • Cytosol / enzymology
  • Gene Expression
  • Genes, Helminth
  • Helminth Proteins / genetics
  • Helminth Proteins / physiology*
  • Longevity / genetics
  • Longevity / physiology*
  • Molecular Sequence Data
  • Mutation
  • Oxidative Stress
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism

Substances

  • CLK-1 protein, C elegans
  • Caenorhabditis elegans Proteins
  • Helminth Proteins
  • Recombinant Fusion Proteins
  • Catalase

Associated data

  • GENBANK/U55384
  • GENBANK/X82175