Interaction between RGS7 and polycystin

Proc Natl Acad Sci U S A. 1999 May 25;96(11):6371-6. doi: 10.1073/pnas.96.11.6371.


Regulators of G protein signaling (RGS) proteins accelerate the intrinsic GTPase activity of certain Galpha subunits and thereby modulate a number of G protein-dependent signaling cascades. Currently, little is known about the regulation of RGS proteins themselves. We identified a short-lived RGS protein, RGS7, that is rapidly degraded through the proteasome pathway. The degradation of RGS7 is inhibited by interaction with a C-terminal domain of polycystin, the protein encoded by PKD1, a gene involved in autosomal-dominant polycystic kidney disease. Furthermore, membranous expression of C-terminal polycystin relocalized RGS7. Our results indicate that rapid degradation and interaction with integral membrane proteins are potential means of regulating RGS proteins.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • B-Lymphocytes / metabolism
  • Binding Sites
  • Cysteine Endopeptidases / metabolism
  • GTP-Binding Proteins / chemistry
  • GTP-Binding Proteins / metabolism
  • Gene Library
  • Humans
  • Molecular Sequence Data
  • Multienzyme Complexes / metabolism
  • Polycystic Kidney, Autosomal Dominant / genetics
  • Polycystic Kidney, Autosomal Dominant / metabolism
  • Proteasome Endopeptidase Complex
  • Protein Biosynthesis
  • Proteins / chemistry
  • Proteins / genetics*
  • Proteins / metabolism*
  • RGS Proteins*
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / metabolism
  • Saccharomyces cerevisiae
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • TRPP Cation Channels
  • Transcription, Genetic
  • Ubiquitins / metabolism


  • Multienzyme Complexes
  • Proteins
  • RGS Proteins
  • RGS7 protein, human
  • Recombinant Proteins
  • TRPP Cation Channels
  • Ubiquitins
  • polycystic kidney disease 1 protein
  • Cysteine Endopeptidases
  • Proteasome Endopeptidase Complex
  • GTP-Binding Proteins

Associated data

  • GENBANK/AF090116
  • GENBANK/AF090117