BLAST 2 Sequences, a new tool for comparing protein and nucleotide sequences

FEMS Microbiol Lett. 1999 May 15;174(2):247-50. doi: 10.1111/j.1574-6968.1999.tb13575.x.


'BLAST 2 Sequences', a new BLAST-based tool for aligning two protein or nucleotide sequences, is described. While the standard BLAST program is widely used to search for homologous sequences in nucleotide and protein databases, one often needs to compare only two sequences that are already known to be homologous, coming from related species or, e.g. different isolates of the same virus. In such cases searching the entire database would be unnecessarily time-consuming. 'BLAST 2 Sequences' utilizes the BLAST algorithm for pairwise DNA-DNA or protein-protein sequence comparison. A World Wide Web version of the program can be used interactively at the NCBI WWW site ( +html). The resulting alignments are presented in both graphical and text form. The variants of the program for PC (Windows), Mac and several UNIX-based platforms can be downloaded from the NCBI FTP site (

MeSH terms

  • Algorithms
  • Amino Acid Sequence*
  • Base Sequence*
  • Sequence Alignment / methods*
  • Software*