Expression of the T-cell markers CD3, CD4 and CD8 in healthy and atopic children during the first 18 months of life

Int Arch Allergy Immunol. 1999 May;119(1):6-12. doi: 10.1159/000024169.


Background: There is little information available about the development of T-cell immunity in healthy and atopic children. We have studied prospectively the mean fluorescence intensity of the T-cell receptor complex-associated CD3, CD4 and CD8 in relation to atopic family history (AFH) and the development of atopic disease.

Methods: Children with a defined AFH (n = 172) were followed from birth to 18 months and the cumulative history of atopic disease was recorded. Blood samples were obtained at birth and at 18 months, and in a subgroup of 78 children also at 3, 6 and 12 months. Multicolour flow cytometry was used to analyse pan T-cells (CD3+CD45+CD14-), T-helper-(CD3+CD4+) and T-cytotoxic-(CD3+CD8+) cells.

Results: At 18 months, 31 children were atopic and 118 non-atopic. Children who developed atopic disease had a higher CD4 expression (mean fluorescence intensity, MFI) on CD4+CD3+ lymphocytes at birth and at 3 months, particularly as compared with non-atopic children without AFH. Furthermore, the CD3 expression on CD3+CD45+CD14- lymphocytes increased more slowly with age in children with double atopic heredity, as compared with children with no or only one atopic family member.

Conclusions: The higher expression of the CD4 receptor in early infancy in children who developed atopic disease compared with non-atopics suggests a delayed expression in T-helper cells. Children with a strong AFH had a slower increase in the expression of CD3, indicating a delayed T-cell maturation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • CD3 Complex / analysis*
  • CD4 Antigens / analysis*
  • CD8 Antigens / analysis*
  • Flow Cytometry
  • Humans
  • Hypersensitivity, Immediate / immunology*
  • Infant
  • Infant, Newborn
  • Lymphocyte Count
  • Prospective Studies
  • Skin Tests
  • T-Lymphocyte Subsets / immunology*


  • CD3 Complex
  • CD4 Antigens
  • CD8 Antigens