The primer generator: a program that facilitates the selection of oligonucleotides for site-directed mutagenesis

Biotechniques. 1999 Apr;26(4):672-6. doi: 10.2144/99264st02.


Site-directed mutagenesis is a powerful tool that has enabled molecular biologists to perform functional analysis of altered nucleic acids and proteins. Newer PCR-based mutagenesis techniques have reduced the process of mutagenesis to as little as one day. While each technique has its advantages, both require a strategy to isolate the desired clone from a population that contains mutagenized and wild-type genes. In this report, we describe a World Wide Web-based computer program that facilitates the design of mutagenic primers such that successfully mutagenized clones can be identified by the presence or absence of a unique restriction site.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Biotechnology
  • DNA Primers / chemical synthesis*
  • DNA Primers / genetics*
  • DNA Restriction Enzymes
  • Drug Design
  • Internet
  • Mutagenesis, Site-Directed*
  • Software Design
  • Software*


  • DNA Primers
  • DNA Restriction Enzymes