Proteins of rat serum IV. Time-course of acute-phase protein expression and its modulation by indomethacine

Electrophoresis. Apr-May 1999;20(4-5):846-53. doi: 10.1002/(SICI)1522-2683(19990101)20:4/5<846::AID-ELPS846>3.0.CO;2-2.

Abstract

Changes in the concentration of major serum proteins were monitored from day 0 to day 4 in three experimental groups: rats injected with turpentine, rats receiving the turpentine shot and daily doses of indomethacine, and rats given indomethacine alone. In inflamed animals, peak changes for acute-phase reactants, evaluated by two-dimensional electrophoresis (2-DE), were usually observed between 48 and 72 h after the phlogistic stimulus. By itself, indomethacine was found to affect the synthesis of most proteins (except one of the thiostatin variants and ceruloplasmin); the changes in serum levels, whether positive or negative, were the same as upon inflammation (except for kallikrein-binding protein), but their extent and/or timing usually differed. When inflamed animals were given indomethacine, a clear-cut difference in the concentration of some proteins was observed versus inflamed rats not given medication, at 24 h after the start of the treatments. Proteins mainly affected were alpha2-macroglobulin, alpha2-HS-glycoprotein, C-reactive protein and kallikrein-binding protein.

MeSH terms

  • Acute-Phase Proteins / metabolism*
  • Animals
  • Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
  • Electrophoresis, Gel, Two-Dimensional
  • Indomethacin / pharmacology*
  • Rats
  • Rats, Sprague-Dawley
  • Time Factors

Substances

  • Acute-Phase Proteins
  • Anti-Inflammatory Agents, Non-Steroidal
  • Indomethacin