Toward an in vitro test for the diagnosis of allergy to penicillins. Synthesis, characterization, and use of beta-lactam and beta-lactam metabolite poly-L-lysines which recognize human IgE antibodies

Bioconjug Chem. 1999 May-Jun;10(3):332-7. doi: 10.1021/bc980023r.

Abstract

Conjugates of poly-L-lysine (PLL) containing a penicillin or a penicilloyl residue were prepared and characterized by 1H NMR and by size-exclusion (SE) HPLC. These conjugates were used in a radio allergo sorbent tests (RAST) test for the determination of allergy toward beta-lactams. The chemiometric evaluation of the data indicates that allergy to amoxycillin is different from allergy to the other beta-lactams tested. Furthermore, careful chemical characterization of the conjugates appears to be crucial to obtain meaningful information from the RAST data.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, High Pressure Liquid
  • Desensitization, Immunologic
  • Drug Hypersensitivity / diagnosis*
  • Drug Hypersensitivity / immunology
  • Humans
  • Immunoglobulin E / immunology*
  • Magnetic Resonance Spectroscopy
  • Penicillins / adverse effects
  • Penicillins / immunology*
  • Polylysine / chemistry
  • Polylysine / immunology*
  • Radioallergosorbent Test
  • beta-Lactams / chemical synthesis
  • beta-Lactams / chemistry
  • beta-Lactams / immunology*

Substances

  • Penicillins
  • beta-Lactams
  • Polylysine
  • Immunoglobulin E