Spatiotemporal dynamics of inositol 1,4,5-trisphosphate that underlies complex Ca2+ mobilization patterns

Science. 1999 May 28;284(5419):1527-30. doi: 10.1126/science.284.5419.1527.


Inositol 1,4,5-trisphosphate (IP3) is a second messenger that elicits complex spatiotemporal patterns of calcium ion (Ca2+) mobilization and has essential roles in the regulation of many cellular functions. In Madin-Darby canine kidney epithelial cells, green fluorescent protein-tagged pleckstrin homology domain translocated from the plasma membrane to the cytoplasm in response to increased concentration of IP3. The detection of translocation enabled monitoring of IP3 concentration changes within single cells and revealed spatiotemporal dynamics in the concentration of IP3 synchronous with Ca2+ oscillations and intracellular and intercellular IP3 waves that accompanied Ca2+ waves. Such changes in IP3 concentration may be fundamental to Ca2+ signaling.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphate / pharmacology
  • Animals
  • Calcium / metabolism*
  • Calcium Signaling*
  • Cell Line
  • Cell Membrane / metabolism
  • Cell Nucleus / metabolism
  • Cytoplasm / metabolism
  • Dogs
  • Green Fluorescent Proteins
  • Inositol 1,4,5-Trisphosphate / metabolism*
  • Inositol Phosphates / metabolism
  • Isoenzymes / chemistry
  • Isoenzymes / metabolism
  • Ligands
  • Luminescent Proteins
  • Microscopy, Confocal
  • Phosphatidylinositol 4,5-Diphosphate / metabolism
  • Phospholipase C delta
  • Recombinant Fusion Proteins / metabolism
  • Time Factors
  • Type C Phospholipases / chemistry
  • Type C Phospholipases / metabolism


  • Inositol Phosphates
  • Isoenzymes
  • Ligands
  • Luminescent Proteins
  • Phosphatidylinositol 4,5-Diphosphate
  • Recombinant Fusion Proteins
  • inositol-1,3,4,5-tetrakisphosphate
  • Green Fluorescent Proteins
  • Inositol 1,4,5-Trisphosphate
  • Adenosine Triphosphate
  • Type C Phospholipases
  • Phospholipase C delta
  • Calcium