A mouse homologue of FAST-1 transduces TGF beta superfamily signals and is expressed during early embryogenesis

Mech Dev. 1998 Dec;79(1-2):17-27. doi: 10.1016/s0925-4773(98)00160-9.


The transcription factor FAST-1 has recently been shown to play a key role in the specification of mesoderm by TGF beta superfamily signals in the early Xenopus embryo. We have cloned Fast1, a mouse homologue of Xenopus FAST-1, and characterized its expression during embryogenesis and function in activin/TGF beta signal transduction. In vitro, Fast1 associates with Smads in response to an activin/TGF beta signal to form a complex that recognizes the Xenopus activin responsive element (ARE) targeted by Xenopus FAST-1. In intact cells, introduction of Fast1 confers activin/TGF beta regulation of an ARE-luciferase reporter. In embryos, Fast1 is expressed predominantly throughout the epiblast before gastrulation and declines as development progresses. We propose that mouse Fast1, like Xenopus FAST-1, mediates TGF beta superfamily signals specifying developmental fate during early embryogenesis.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cloning, Molecular
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism
  • Forkhead Transcription Factors
  • Gastrula / physiology
  • Gene Expression Regulation, Developmental*
  • Humans
  • Mice / embryology*
  • Nerve Growth Factors
  • Sequence Homology, Amino Acid
  • Signal Transduction
  • Smad Proteins
  • Smad2 Protein
  • Smad3 Protein
  • Smad4 Protein
  • Trans-Activators / genetics
  • Trans-Activators / metabolism
  • Transcription Factors / genetics*
  • Transcription Factors / metabolism
  • Transcription, Genetic
  • Transforming Growth Factor beta / metabolism*
  • Xenopus
  • Xenopus Proteins*


  • DNA-Binding Proteins
  • FOXH1 protein, Xenopus
  • FOXH1 protein, human
  • Forkhead Transcription Factors
  • Foxh1 protein, mouse
  • Nerve Growth Factors
  • SMAD2 protein, human
  • SMAD3 protein, human
  • SMAD4 protein, human
  • Smad Proteins
  • Smad2 Protein
  • Smad2 protein, Xenopus
  • Smad2 protein, mouse
  • Smad3 Protein
  • Smad3 protein, mouse
  • Smad4 Protein
  • Smad4 protein, mouse
  • Trans-Activators
  • Transcription Factors
  • Transforming Growth Factor beta
  • Xenopus Proteins
  • madh3 protein, Xenopus
  • smad4.1 protein, Xenopus
  • smad4.2 protein, Xenopus