Rpn4p acts as a transcription factor by binding to PACE, a nonamer box found upstream of 26S proteasomal and other genes in yeast

FEBS Lett. 1999 Apr 30;450(1-2):27-34. doi: 10.1016/s0014-5793(99)00467-6.


We identified a new, unique upstream activating sequence (5'-GGTGGCAAA-3') in the promoters of 26 out of the 32 proteasomal yeast genes characterized to date, which we propose to call proteasome-associated control element. By using the one-hybrid method, we show that the factor binding to the proteasome-associated control element is Rpn4p, a protein containing a C2H2-type finger motif and two acidic domains. Electrophoretic mobility shift assays using proteasome-associated control element sequences from two regulatory proteasomal genes confirmed specific binding of purified Rpn4p to these sequences. The role of Rpn4p to function as a transregulator in yeast is corroborated by its ability of stimulating proteasome-associated control element-driven lacZ expression and by experiments using the RPT4 and RPT6 gene promoters coupled to the bacterial cat gene as a reporter. Additionally, we found the proteasome-associated control element to occur in a number of promoters to genes which are related to the ubiquitin-proteasome pathway in yeast.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Binding Sites
  • Cloning, Molecular
  • Cysteine Endopeptidases / genetics*
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / genetics*
  • Gene Expression Regulation, Fungal
  • Genes, Fungal
  • Genes, Reporter
  • Molecular Sequence Data
  • Multienzyme Complexes / genetics*
  • Oligodeoxyribonucleotides / genetics
  • Promoter Regions, Genetic*
  • Proteasome Endopeptidase Complex
  • Regulatory Sequences, Nucleic Acid
  • Saccharomyces cerevisiae Proteins*
  • Transcription Factors / chemistry
  • Transcription Factors / genetics*
  • Yeasts / enzymology*
  • Zinc Fingers / genetics


  • DNA-Binding Proteins
  • Multienzyme Complexes
  • Oligodeoxyribonucleotides
  • RPN4 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • Cysteine Endopeptidases
  • Proteasome Endopeptidase Complex