Molecular cloning and expression of a novel human cDNA related to the diazepam binding inhibitor

Biochim Biophys Acta. 1999 May 31;1454(1):126-31. doi: 10.1016/s0925-4439(99)00033-2.


In order to isolate the unidentified autoantigens in autoimmune diabetes, a human pancreatic islet cDNA library was constructed and screened with the sera from the diabetic patients. From the library screening, one clone (DRS-1) that strongly reacted with the sera was isolated. Subsequent sequence analysis revealed that the clone was a novel cDNA related to the diazepam binding inhibitor. DRS-1 was expressed in most tissues including liver, lung, tonsil, and thymus, in addition to pancreatic islets. DRS-1 was in vitro translated and the recombinant DRS-1 protein was expressed in Escherichia coli and purified. The size of the in vitro translated or bacterially expressed DRS-1 protein was in agreement with the conceptually translated polypeptide of DRS-1 cDNA. Further studies are required to test whether or not DRS-1 is a new autoantigen in autoimmune diabetes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Autoantigens / genetics
  • Base Sequence
  • Carbon-Carbon Double Bond Isomerases
  • Cloning, Molecular
  • DNA, Complementary / biosynthesis*
  • DNA, Complementary / chemistry
  • DNA, Complementary / isolation & purification
  • Diabetes Mellitus, Type 1 / genetics
  • Dodecenoyl-CoA Isomerase
  • Gene Library
  • Humans
  • Islets of Langerhans / metabolism
  • Isomerases*
  • Molecular Sequence Data
  • RNA / isolation & purification
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / genetics*
  • Sequence Alignment


  • Autoantigens
  • DNA, Complementary
  • Recombinant Proteins
  • RNA
  • Isomerases
  • Carbon-Carbon Double Bond Isomerases
  • Dodecenoyl-CoA Isomerase
  • ECI2 protein, human

Associated data

  • GENBANK/AF069301