Detection of ileS-2 Gene Encoding Mupirocin Resistance in Methicillin-Resistant Staphylococcus Aureus by Multiplex PCR

Diagn Microbiol Infect Dis. 1999 Jun;34(2):77-81. doi: 10.1016/s0732-8893(99)00021-8.

Abstract

The presence of the ileS-2 gene, responsible for mupirocin resistance, in clinical isolates of methicillin-resistant Staphylococcus aureus was determined by multiplex polymerase chain reaction. Three pairs of primers were used, which yielded specific fragments of femA (encoding a unique feature of S. aureus), mecA (encoding resistance to methicillin) and ileS-2 genes. The multiplex polymerase chain reaction system is an easy and time-saving technique that, together with a rapid method for DNA extraction by boiling, may be incorporated as a routine analysis in clinical diagnostic laboratories.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Bacterial Proteins / genetics
  • DNA Primers
  • Drug Resistance, Microbial / genetics*
  • Genes, Bacterial*
  • Humans
  • Methicillin Resistance / genetics*
  • Mupirocin / pharmacology*
  • Polymerase Chain Reaction / methods
  • Staphylococcal Infections / microbiology
  • Staphylococcus aureus / drug effects*
  • Staphylococcus aureus / genetics

Substances

  • Anti-Bacterial Agents
  • Bacterial Proteins
  • DNA Primers
  • FemA protein, Bacteria
  • Mupirocin