Mucosal immunization of calves with recombinant bovine adenovirus-3: induction of protective immunity to bovine herpesvirus-1

J Gen Virol. 1999 May;80 ( Pt 5):1263-1269. doi: 10.1099/0022-1317-80-5-1263.

Abstract

To determine the potential of replication-competent (E3-deleted) bovine adenovirus-3 (BAV-3) as a delivery system for vaccine antigens in calves, we evaluated the ability of recombinant BAV-3 expressing different forms of of bovine herpesvirus-1 (BHV-1) glycoprotein gD to protect against BHV-1 infection in calves that had pre-existing BAV-3 specific antibodies. Three- to four-month-old calves, vaccinated intranasally with recombinant BAV-3 expressing full-length gD (BAV3.E3gD) or a truncated version of gD (gDt) (BAV3.E3gDt), or with E3-deleted BAV-3 (BAV3.E3d; control), were challenged with BHV-1 strain 108. Vaccination with BAV3.E3gD or BAV3.E3gDt induced gD-specific antibody responses in serum and nasal secretions, and primed calves for gD-specific lymphoproliferative responses. In addition, all calves developed complement-independent neutralizing antibodies against BHV-1. Protection against viral challenge was observed in calves vaccinated with recombinant BAV3.E3gD or BAV3.E3gDt as shown by a significant reduction in body temperature and clinical disease, and a partial reduction in the amount and duration of virus excretion in nasal secretions. These results indicate that replication-competent BAV-3-based vectors can induce protective immune responses in calves (the natural host) that have pre-existing BAV-3-specific antibodies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenovirus E3 Proteins / genetics*
  • Adenovirus E3 Proteins / immunology
  • Animals
  • Antibodies, Viral / blood
  • Antigens, Viral / immunology
  • Cattle
  • Cattle Diseases / immunology
  • Cattle Diseases / prevention & control
  • Genetic Vectors
  • Herpesviridae Infections / immunology
  • Herpesviridae Infections / prevention & control
  • Herpesviridae Infections / veterinary*
  • Herpesvirus 1, Bovine / immunology*
  • Immunity, Mucosal
  • Immunization
  • Mastadenovirus / genetics
  • Mastadenovirus / immunology*
  • Recombinant Proteins / immunology
  • Vaccines, Synthetic / administration & dosage
  • Vaccines, Synthetic / immunology
  • Viral Proteins / immunology*
  • Viral Vaccines / administration & dosage
  • Viral Vaccines / immunology*

Substances

  • Adenovirus E3 Proteins
  • Antibodies, Viral
  • Antigens, Viral
  • Recombinant Proteins
  • Vaccines, Synthetic
  • Viral Proteins
  • Viral Vaccines
  • bovine herpesvirus type-1 glycoproteins