Interactions between 192-IgG saporin and intraseptal cholinergic and GABAergic drugs: role of cholinergic medial septal neurons in spatial working memory

Behav Neurosci. 1999 Apr;113(2):265-75. doi: 10.1037//0735-7044.113.2.265.


Rats were administered 192-IgG saporin (SAP) or vehicle into the medial septum-vertical limb of the diagonal band (MS-vDB). Starting 1 week later, the effects of intraseptal scopolamine, oxotremorine, and muscimol were tested in a T-maze alternation task. Choice accuracy in the absence of infusions did not differ between control and SAP-treated rats. Intraseptal scopolamine or muscimol impaired the choice accuracy of SAP-treated but not control rats. Oxotremorine impaired accuracy similarly in control and SAP-treated rats. The enhanced effects of scopolamine and muscimol produced by SAP are consistent with the hypothesis that cholinergic MS-vDB neurons are used in spatial working memory. The finding that SAP alone did not alter choice accuracy provides further evidence that cholinergic MS-vDB neurons are not necessary for spatial working memory. Thus, cholinergic MS-vDB neurons are involved in but not necessary for spatial working memory.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / pharmacology*
  • Cholinergic Agents / pharmacology*
  • Drug Interactions
  • Frontal Lobe / drug effects
  • Frontal Lobe / pathology
  • Frontal Lobe / physiology*
  • GABA Agents / pharmacology*
  • Immunotoxins / pharmacology*
  • Injections, Intraventricular
  • Male
  • Maze Learning / drug effects
  • Maze Learning / physiology
  • Memory / drug effects*
  • Memory / physiology*
  • Muscimol / pharmacology
  • N-Glycosyl Hydrolases
  • Neurons, Afferent / drug effects
  • Oxotremorine / pharmacology
  • Rats
  • Rats, Inbred F344
  • Ribosome Inactivating Proteins, Type 1
  • Saporins
  • Scopolamine / pharmacology
  • Space Perception / drug effects
  • Space Perception / physiology


  • 192 IgG-saporin
  • Antibodies, Monoclonal
  • Cholinergic Agents
  • GABA Agents
  • Immunotoxins
  • Ribosome Inactivating Proteins, Type 1
  • Muscimol
  • Oxotremorine
  • Scopolamine
  • N-Glycosyl Hydrolases
  • Saporins