Acquisition of fear conditioning in rats requires the synthesis of mRNA in the amygdala

Behav Neurosci. 1999 Apr;113(2):276-82. doi: 10.1037//0735-7044.113.2.276.


In this study, the role of mRNA synthesis in the amygdala was studied during the acquisition of conditional fear. Rats with cannulas placed in the basolateral region of the amygdala were trained with a series of noise-shock pairings in a distinctive observation chamber. One half of the rats were pretreated with the mRNA synthesis inhibitor actinomycin-D (act-D). Responding to the training context and the auditory stimulus in a novel context measured by defensive freezing was assessed. Pretreatment with act-D significantly attenuated fear responses to both stimuli. Animals receiving act-D injections exhibited normal reactions to the conditioned stimulus-unconditioned stimulus pairings in the initial training session and displayed normal learning when retrained 7 days after injections. These results indicate that the transcription of new mRNA and subsequent protein synthesis in the amygdala may be essential for neural plasticity during this form of associative learning.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amygdala / drug effects
  • Amygdala / metabolism*
  • Animals
  • Association Learning / physiology*
  • Conditioning, Classical* / drug effects
  • Dactinomycin / pharmacology
  • Fear*
  • Male
  • Memory, Short-Term / drug effects
  • Memory, Short-Term / physiology
  • Neuronal Plasticity
  • Nucleic Acid Synthesis Inhibitors / pharmacology
  • RNA, Messenger / antagonists & inhibitors
  • RNA, Messenger / biosynthesis*
  • Rats
  • Rats, Long-Evans


  • Nucleic Acid Synthesis Inhibitors
  • RNA, Messenger
  • Dactinomycin