Limb allotransplantation in rats: combined immunosuppression by FK-506 and 15-deoxyspergualin

J Hand Surg Am. 1999 May;24(3):586-93. doi: 10.1053/jhsu.1999.0586.


The immunosuppressive effect of combined therapy using FK-506 and 15-deoxyspergualin was investigated in rat limb allotransplantation. The right hindlimb of an inbred Dark Agouti rat (RT1a) was transplanted to a Lewis rat (RT1l) and observed for 120 days. Eighty-eight transplantations were performed. Rejection of the grafts was evaluated histologically as well as macroscopically. A pathologic grading system was used to rate the severity of rejection. Limb survival time in Lewis rats receiving FK-506 therapy for days was significantly prolonged by combined therapy with 15-deoxyspergualin. Histologic study showed practically no rejection in all the graft-composing tissues except the skin and bone marrow, in which there was rejection. These results suggest that tissues like bone, cartilage, and muscle may survive transplantation with short-term combination FK-506 and 15-deoxyspergualin therapy even after withdrawal of both agents.

MeSH terms

  • Animals
  • Drug Therapy, Combination
  • Femur / pathology
  • Graft Survival*
  • Guanidines / therapeutic use*
  • Hindlimb / transplantation*
  • Immunosuppressive Agents / therapeutic use*
  • Muscle, Skeletal / pathology
  • Rats
  • Rats, Inbred Lew
  • Skin / pathology
  • Tacrolimus / therapeutic use*
  • Transplantation, Homologous


  • Guanidines
  • Immunosuppressive Agents
  • gusperimus
  • Tacrolimus