Abstract
The budding yeast Saccharomyces cerevisiae has been the principal organism used in experiments to examine genetic recombination in eukaryotes. Studies over the past decade have shown that meiotic recombination and probably most mitotic recombination arise from the repair of double-strand breaks (DSBs). There are multiple pathways by which such DSBs can be repaired, including several homologous recombination pathways and still other nonhomologous mechanisms. Our understanding has also been greatly enriched by the characterization of many proteins involved in recombination and by insights that link aspects of DNA repair to chromosome replication. New molecular models of DSB-induced gene conversion are presented. This review encompasses these different aspects of DSB-induced recombination in Saccharomyces and attempts to relate genetic, molecular biological, and biochemical studies of the processes of DNA repair and recombination.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
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Review
MeSH terms
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Adenosine Triphosphatases
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Animals
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DNA Damage / genetics
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DNA Helicases
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DNA Repair / genetics
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DNA Repair / physiology*
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DNA Repair Enzymes
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DNA Replication / physiology
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DNA, Fungal / genetics*
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DNA-Binding Proteins / physiology
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Deoxyribonucleases, Type II Site-Specific / physiology
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Fungal Proteins / physiology
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Humans
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Meiosis
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Rad52 DNA Repair and Recombination Protein
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Rec A Recombinases / metabolism
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Recombination, Genetic / genetics*
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae Proteins*
Substances
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DNA, Fungal
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DNA-Binding Proteins
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Fungal Proteins
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RAD52 protein, S cerevisiae
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RAD55 protein, S cerevisiae
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Rad52 DNA Repair and Recombination Protein
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Saccharomyces cerevisiae Proteins
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Rec A Recombinases
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SCEI protein, S cerevisiae
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Deoxyribonucleases, Type II Site-Specific
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Adenosine Triphosphatases
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RAD54 protein, S cerevisiae
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RAD57 protein, S cerevisiae
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DNA Helicases
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DNA Repair Enzymes