Abstract
Fetal liver- and thymus-derived NK1.1+ cells do not express known Ly-49 receptors. Despite the absence of Ly-49 inhibitory receptors, fetal and neonatal NK1.1+Ly-49- cells can distinguish between class Ihigh and class Ilow target cells, suggesting the existence of other class I-specific inhibitory receptors. We demonstrate that fetal NK1. 1+Ly-49- cell lysates contain CD94 protein and that a significant proportion of fetal NK cells are bound by Qa1b tetramers. Fetal and adult NK cells efficiently lyse lymphoblasts from Kb-/-Db-/- mice. Qa1b-specific peptides Qdm and HLA-CW4 leader peptide specifically inhibited the lysis of these blasts by adult and fetal NK cells. Qdm peptide also inhibited the lysis of Qa1b-transfected human 721.221 cells by fetal NK cells. Taken together, these results suggest that the CD94/NKG2A receptor complex is the major known inhibitory receptor for class I (Qa1b) molecules on developing fetal NK cells.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Aging / immunology
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Amino Acid Sequence
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Animals
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Antigens / biosynthesis*
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Antigens, CD / biosynthesis*
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Antigens, CD / genetics
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Antigens, CD / immunology
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Antigens, Ly*
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Antigens, Surface
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Cell Differentiation / genetics
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Cell Differentiation / immunology
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Cell-Free System / immunology
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Cells, Cultured
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Cytotoxicity, Immunologic / drug effects
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Cytotoxicity, Immunologic / genetics
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Fetus
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Histocompatibility Antigens Class I / biosynthesis
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Histocompatibility Antigens Class I / immunology
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Histocompatibility Antigens Class I / metabolism
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Immune Sera / chemistry
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Immune Tolerance* / genetics
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Killer Cells, Natural / cytology*
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Killer Cells, Natural / immunology
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Killer Cells, Natural / metabolism*
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Lectins, C-Type*
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Liver / cytology
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Liver / immunology
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Lymphocyte Activation / drug effects
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Lymphocyte Activation / genetics
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Membrane Glycoproteins / biosynthesis*
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Membrane Glycoproteins / genetics
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Membrane Glycoproteins / immunology
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Mice
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Mice, Knockout
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Molecular Sequence Data
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NK Cell Lectin-Like Receptor Subfamily B
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NK Cell Lectin-Like Receptor Subfamily D
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Peptides / immunology
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Peptides / pharmacology
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Protein Binding / immunology
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Protein Biosynthesis*
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Proteins*
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Receptors, NK Cell Lectin-Like
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Spleen / cytology
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Spleen / growth & development
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Spleen / immunology
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T-Lymphocytes / immunology
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Thymus Gland / cytology
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Thymus Gland / immunology
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Transcription, Genetic / immunology
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Transfection / immunology
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Tumor Cells, Cultured
Substances
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Antigens
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Antigens, CD
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Antigens, Ly
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Antigens, Surface
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Histocompatibility Antigens Class I
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Immune Sera
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KLRB1 protein, human
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Klrb1c protein, mouse
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Klrd1 protein, mouse
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Lectins, C-Type
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Membrane Glycoproteins
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NK Cell Lectin-Like Receptor Subfamily B
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NK Cell Lectin-Like Receptor Subfamily D
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Peptides
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Proteins
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Q surface antigens
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Receptors, NK Cell Lectin-Like