A bacteriophage encoding a pathogenicity island, a type-IV pilus and a phage receptor in cholera bacteria

Nature. 1999 May 27;399(6734):375-9. doi: 10.1038/20715.

Abstract

The virulence properties of many pathogenic bacteria are due to proteins encoded by large gene clusters called pathogenicity islands, which are found in a variety of human pathogens including Escherichia coli, Salmonella, Shigella, Yersinia, Helicobacter pylori, Vibrio cholerae, and animal and plant pathogens such as Dichelobacter nodosus and Pseudomonas syringae. Although the presence of pathogenicity islands is a prerequisite for many bacterial diseases, little is known about their origins or mechanism of transfer into the bacterium. The bacterial agent of epidemic cholera, Vibrio cholerae, contains a bacteriophage known as cholera-toxin phage (CTXphi), which encodes the cholera toxin, and a large pathogenicity island called the VPI (for V. cholerae pathogenicity island) which itself encodes a toxin-coregulated pilus that functions as a colonization factor and as a CTXphi receptor. We have now identified the VPI pathogenicity island as the genome of another filamentous bacteriophage, VPIphi. We show that VPIphi is transferred between V. cholerae strains and provide evidence that the TcpA subunit of the toxin-coregulated type IV pilus is in fact a coat protein of VPIphi. Our results are the first description of a phage that encodes a receptor for another phage and of a virus-virus interaction that is necessary for bacterial pathogenicity.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bacterial Outer Membrane Proteins / genetics
  • Bacteriophages / genetics*
  • Bacteriophages / isolation & purification
  • Bacteriophages / physiology
  • Cholera Toxin / genetics*
  • Fimbriae Proteins*
  • Fimbriae, Bacterial / genetics*
  • Genes, Viral
  • Multigene Family
  • Plasmids
  • Polymerase Chain Reaction
  • Receptors, Virus / genetics*
  • Transduction, Genetic
  • Vibrio cholerae / pathogenicity
  • Vibrio cholerae / virology*
  • Virus Replication

Substances

  • Bacterial Outer Membrane Proteins
  • Receptors, Virus
  • TcpA protein, Vibrio cholerae
  • Fimbriae Proteins
  • Cholera Toxin