CKSHS Expression Is Linked to Cell Proliferation in Normal and Malignant Human Lymphoid Cells

Int J Cancer. 1999 Jul 2;82(1):98-104. doi: 10.1002/(sici)1097-0215(19990702)82:1<98::aid-ijc17>3.0.co;2-a.

Abstract

Cyclin kinase sub-units (CKS) are known to interact with cyclin-dependent kinases (CDKs), but their functions are not completely understood and their expression in human tissues is not documented. For analyzing relationships of CKS with cell proliferation and/or with differentiation, we investigated the expression of ckshs1 and ckshs2 in normal and malignant human lymphoid cells. ckshs1 and ckshs2 expression appeared to be related to cell proliferation: (i) mRNAs increased with stimulation of normal peripheral-blood lymphocytes, and from the G1 to the SG2M phase in elutriated cells; (ii) P9 proteins were also induced by lymphocyte stimulation and were localized in nucleus where phosphorylated forms of CDK1 were also found; (iii) in vitro, the phosphorylated forms of CDK1 and CDK2 were preferentially linked to CKS. Among 45 patients presenting acute or chronic lymphoid malignancy, ckshs1 and ckshs2 mRNAs varied in a similar way and were significantly correlated to cell proliferation (p < 0.0001). When analysis was restricted solely to acute lymphoblastic leukemia (ALL) this correlation was still found and ckshs1 and ckshs2 were significantly more expressed in T-cell ALL than in B-cell-lineage ALL. These results confirm relationships between ckshs expression and cell proliferation, and pose the question of a link with cell differentiation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CDC2-CDC28 Kinases
  • Carrier Proteins / genetics*
  • Cell Cycle
  • Cell Cycle Proteins*
  • Cell Division
  • Cell Line
  • Cyclin-Dependent Kinases
  • Humans
  • Lymphocytes / metabolism*
  • Precursor Cell Lymphoblastic Leukemia-Lymphoma / metabolism*
  • Precursor Cell Lymphoblastic Leukemia-Lymphoma / pathology
  • Protein Kinases*
  • RNA, Messenger / analysis
  • Rabbits

Substances

  • CKS1B protein, human
  • Carrier Proteins
  • Cell Cycle Proteins
  • RNA, Messenger
  • Protein Kinases
  • CDC2-CDC28 Kinases
  • CKS2 protein, human
  • Cyclin-Dependent Kinases