The choice of reference cell in the analysis of kinetic data using BIAcore

Anal Biochem. 1999 Jun 15;271(1):70-80. doi: 10.1006/abio.1999.4129.

Abstract

An important step in the analysis of sensorgram data for BIAcore experiments is the subtraction of reference cell data to remove the effects of the bulk shift on the sensorgram of interest. It is shown that this step can introduce errors in the measured kinetic constants. This phenomenon is investigated both theoretically and with experimental data.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / metabolism
  • Antigen-Antibody Reactions
  • Chickens
  • Data Interpretation, Statistical
  • Kinetics
  • Muramidase / immunology
  • Muramidase / metabolism
  • Reference Standards
  • Surface Plasmon Resonance / instrumentation*
  • Surface Plasmon Resonance / standards
  • Surface Plasmon Resonance / statistics & numerical data

Substances

  • Antibodies, Monoclonal
  • Muramidase