Monitoring the Growth of a Bacteria Culture by MALDI-MS of Whole Cells

Anal Chem. 1999 May 15;71(10):1990-6. doi: 10.1021/ac981196c.

Abstract

We have probed the time evolution of a growing bacteria culture by extracting samples periodically and performing matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) on whole cells. The mass spectra generated by this method contain tens of peaks in the 3-11-kDa mass range. Cultures of E. coli strain K-12 were grown in two types of containers and at two nutrient concentrations and sampled periodically from 6 to 84 h after inoculation. The relative intensities of several of the stronger peaks vary quite dramatically as a function of time. These temporal characteristics must be taken into account when MALDI-MS is applied to identify bacteria. The results also suggest that MALDI-MS can be used to follow the aging of a bacteria culture.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Escherichia coli / chemistry*
  • Escherichia coli / growth & development*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization*