Objective: To study the alterations in body weight, tissue weight and total protein in mice, caused by a single sublethal injection of arsenic trioxide and to investigate whether treatment by microdoses of arsenic has any antidotal effect.
Methods: For each fixation interval, altogether 36 individuals of Swiss albino mice, Mus musculus, were used, 27 were injected with As2O3 in a single sub-lethal dose (@1.0 mg/kg body weight) and were divided into three batches. One batch was fed with diluted potentized alcohol (Alcohol control), one batch was fed with potentized homoeopathic drug Ars.Alb-30 (Active treatment), while the remaining one neither fed with potenized alcohol nor with the potentized homoeopathic drug (As-intoxicated control). The remaining batch of nine mice were injected with normal saline which served as negative control (Saline control). The mean body weights before and after injections and weights of different tissues like liver, kidney, spleen and testis were recorded at seven fixation intervals, 12 hours, 24 hours, 48 hours, 7 days, 21 days, 30 days, and 90 days.
Results: In arsenic treated mice orally administered with the homoeopathic drug statistically significant increases were noted in the weights of individual tissue weight, protein content as well as the mean body weight as compared to their respective controls.
Conclusions: Arsenicum album can be considered as an antidote to arsenic poisoning.