A nonhuman primate model for the selective elimination of CD8+ lymphocytes using a mouse-human chimeric monoclonal antibody

Am J Pathol. 1999 Jun;154(6):1923-32. doi: 10.1016/S0002-9440(10)65450-8.


Nonhuman primates provide valuable animal models for human diseases. However, studies assessing the role of cell-mediated immune responses have been difficult to perform in nonhuman primates. We have shown that CD8+ lymphocyte-mediated immunity in rhesus monkeys can be selectively eliminated using the mouse-human chimeric anti-CD8 monoclonal antibody cM-T807. In vitro, this antibody completely blocked antigen-specific expansion of cytotoxic T cells and decreased major histocompatibility complex class I-restricted, antigen-specific lysis of target cells but did not mediate complement-dependent cell lysis. In vivo administration of cM-T807 in rhesus monkeys resulted in near total depletion of CD8+ T cells from the blood and lymph nodes for up to 6 weeks. This depletion was not solely complement-dependent and persisted longer in adults than in juveniles. Preservation of B cell and CD4+ T cell function in monkeys depleted of CD8+ lymphocytes was demonstrated by their ability to develop humoral immune responses to the administered chimeric monoclonal antibody. Furthermore, during CD8+ lymphocyte depletion, monkeys developed delayed-type hypersensitivity reactions comprised only of CD4+ T cells but not CD8+ T cells. This CD8+ lymphocyte depletion model should prove useful in defining the role of cell-mediated immune responses in controlling infectious diseases in nonhuman primates.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies / blood
  • Antibodies, Monoclonal / administration & dosage*
  • Antibody Formation / drug effects
  • Antigens, CD / immunology
  • CD8-Positive T-Lymphocytes / cytology*
  • CD8-Positive T-Lymphocytes / drug effects*
  • CD8-Positive T-Lymphocytes / immunology
  • Complement Activation / drug effects
  • Elapid Venoms / pharmacology
  • Humans
  • Hypersensitivity, Delayed / blood
  • Hypersensitivity, Delayed / immunology
  • Immunity, Cellular / drug effects
  • Lymphocyte Depletion*
  • Macaca mulatta
  • Mice
  • Models, Immunological*
  • Recombinant Fusion Proteins / administration & dosage*
  • Simian Acquired Immunodeficiency Syndrome / immunology
  • Tetanus Toxoid / immunology


  • Antibodies
  • Antibodies, Monoclonal
  • Antigens, CD
  • Elapid Venoms
  • Recombinant Fusion Proteins
  • Tetanus Toxoid
  • cobra venom factor