Characterization of a novel receptor that maps near the natural killer gene complex: demonstration of carbohydrate binding and expression in hematopoietic cells

Cancer Res. 1999 Jun 1;59(11):2709-17.


A novel type II integral membrane protein has been identified in the course of screening for genes overexpressed in a mouse model of chronic myelogenous leukemia blast crisis. This new protein, designated NKCL, consists of a 210-amino acid polypeptide with a short, NH2-terminal cytoplasmic tail of 17 amino acids preceding a transmembrane domain and a COOH-terminal extracellular region. The COOH-terminal 132 amino acids bear typical features of the C-type animal lectin carbohydrate-recognition domain. The Nkcl gene is unique in that it maps just proximal to the region of the genome that encodes group V members of the C-type animal lectin family near the natural killer gene complex on mouse chromosome 6, but its protein product also has features of several group II C-type animal lectins. Most notably, it has a complete Ca2+-binding site 2, which forms part of the sugar-binding site in other members of the family, and binds mannose in a Ca2+-dependent manner. Moreover, its expression is not restricted to natural killer cells, as reported for the majority of group V lectins. Nkcl is expressed in pluripotent myeloid precursors, precursor and mature macrophages, and neutrophils.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blast Crisis / genetics*
  • Blotting, Northern
  • COS Cells
  • Chromosome Mapping
  • Genetic Vectors / genetics
  • Hematopoietic Stem Cells / metabolism*
  • Killer Cells, Natural*
  • Lectins / genetics*
  • Lectins / metabolism
  • Lectins, C-Type*
  • Leukemia, Myelogenous, Chronic, BCR-ABL Positive / genetics*
  • Membrane Proteins / genetics*
  • Membrane Proteins / metabolism
  • Mice
  • Microscopy, Fluorescence
  • Molecular Sequence Data
  • Neoplasm Proteins / genetics*
  • Neoplasm Proteins / metabolism
  • Receptors, Cell Surface / genetics*
  • Receptors, Cell Surface / metabolism
  • Transfection


  • Clec4n protein, mouse
  • Lectins
  • Lectins, C-Type
  • Membrane Proteins
  • Neoplasm Proteins
  • Receptors, Cell Surface