Identification of a cis-regulatory element involved in phytochrome down-regulated expression of the pea small GTPase gene pra2

Plant Physiol. 1999 Jun;120(2):491-500. doi: 10.1104/pp.120.2.491.


The pra2 gene encodes a pea (Pisum sativum) small GTPase belonging to the YPT/rab family, and its expression is down-regulated by light, mediated by phytochrome. We have isolated and characterized a genomic clone of this gene and constructed a fusion DNA of its 5'-upstream region in front of the gene for firefly luciferase. Using this construct in a transient assay, we determined a pra2 cis-regulatory region sufficient to direct the light down-regulation of the luciferase reporter gene. Both 5'- and internal deletion analyses revealed that the 93-bp sequence between -734 and -642 from the transcriptional start site was important for phytochrome down-regulation. Gain-of-function analysis showed that this 93-bp region could confer light down-regulation when fused to the cauliflower mosaic virus 35S promoter. Furthermore, linker-scanning analysis showed that a 12-bp sequence within the 93-bp region mediated phytochrome down-regulation. Gel-retardation analysis showed the presence of a nuclear factor that was specifically bound to the 12-bp sequence in vitro. These results indicate that this element is a cis-regulatory element involved in phytochrome down-regulated expression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • Cotyledon / enzymology
  • DNA Primers / genetics
  • DNA-Binding Proteins / metabolism
  • Down-Regulation / radiation effects
  • GTP Phosphohydrolases / genetics*
  • GTP-Binding Proteins / genetics*
  • Gene Expression Regulation, Enzymologic / radiation effects
  • Gene Expression Regulation, Plant / radiation effects
  • Genes, Plant*
  • Genes, Regulator
  • Genes, Reporter
  • Light
  • Molecular Sequence Data
  • Peas / enzymology*
  • Peas / genetics*
  • Peas / radiation effects
  • Phytochrome / metabolism
  • Plant Proteins / genetics*
  • Plant Proteins / metabolism
  • Promoter Regions, Genetic
  • rab GTP-Binding Proteins*


  • DNA Primers
  • DNA-Binding Proteins
  • Plant Proteins
  • Phytochrome
  • GTP Phosphohydrolases
  • GTP-Binding Proteins
  • PRA2 protein, Pisum sativum
  • rab GTP-Binding Proteins

Associated data

  • GENBANK/AB007911