Synthesis of basement membrane by gastrointestinal cancer cell lines

J Pathol. 1999 Jan;187(2):223-8. doi: 10.1002/(SICI)1096-9896(199901)187:2<223::AID-PATH219>3.0.CO;2-B.

Abstract

Gastrointestinal adenocarcinoma-derived cell lines were studied in order to determine their pattern of expression of basement membrane components and their ability to form a basement membrane. In contrast to the well-preserved expression of laminin beta 2, beta 3, gamma 1, and gamma 2 chain mRNAs, five of eight gastrointestinal cancer cells lacked alpha 3 mRNA. Immunohistochemical and electron microscopic examination of four cell lines transplanted subcutaneously to SCID mice demonstrated the presence of both alpha 3 and alpha 5 chains and the formation of a basal lamina in two cases. The other two cell lines lacked both alpha 3 and alpha 5 chains and could not form a basal lamina, suggesting that this deficiency may be a factor which affects their ability to form a basement membrane. This abnormality might play some role in stromal invasion by tumour cells in gastrointestinal cancer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Basement Membrane / metabolism*
  • Basement Membrane / ultrastructure
  • Blotting, Northern
  • Gastrointestinal Neoplasms / metabolism*
  • Gastrointestinal Neoplasms / ultrastructure
  • Gene Expression
  • Humans
  • Laminin / biosynthesis*
  • Laminin / genetics
  • Mice
  • Mice, SCID
  • Neoplasm Proteins / biosynthesis*
  • Neoplasm Proteins / genetics
  • Neoplasm Transplantation
  • RNA, Messenger / genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Tumor Cells, Cultured

Substances

  • Laminin
  • Neoplasm Proteins
  • RNA, Messenger