cDNA and structural organization of the gene Pole1 for the mouse DNA polymerase epsilon catalytic subunit

Biochim Biophys Acta. 1999 Jun 9;1445(3):363-71. doi: 10.1016/s0167-4781(99)00058-5.


The cDNA and the gene for the mouse DNA polymerase epsilon catalytic subunit were cloned. The deduced protein sequence shows remarkable evolutionary conservation in DNA polymerase epsilon family. However, several conserved elements involved in template-primer binding differ from those of other class B polymerases. This is likely to reflect a distinctive function of the enzyme. The gene that was assigned to chromosome 5 region E3-E5, consists of 49 exons and has a non-conforming splice site in the junction of exon and intron 13. A CpG island covers the promoter region which contains several putative consensus elements critical for S phase upregulated and serum responsive promoters.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Binding Sites
  • Catalysis
  • DNA Polymerase II / chemistry*
  • DNA Polymerase II / genetics*
  • DNA, Complementary / chemistry
  • Exons
  • Introns
  • Mice
  • Molecular Sequence Data
  • Poly-ADP-Ribose Binding Proteins


  • DNA, Complementary
  • Poly-ADP-Ribose Binding Proteins
  • DNA Polymerase II
  • Pole protein, mouse

Associated data

  • GENBANK/AF123502
  • GENBANK/AF126377
  • GENBANK/AF126378
  • GENBANK/AF126379
  • GENBANK/AF126380
  • GENBANK/AF126381
  • GENBANK/AF126382
  • GENBANK/AF126383
  • GENBANK/AF126384
  • GENBANK/AF126385
  • GENBANK/AF126386
  • GENBANK/AF126387
  • GENBANK/AF126388
  • GENBANK/AF126389
  • GENBANK/AF126390
  • GENBANK/AF126391
  • GENBANK/AF126392
  • GENBANK/AF126393
  • GENBANK/AF126394
  • GENBANK/AF126395
  • GENBANK/AF126396
  • GENBANK/AF126397
  • GENBANK/AF126398
  • GENBANK/AH008043