Association of the interleukin-4 receptor alpha chain with p47phox, an activator of the phagocyte NADPH oxidase in B cells

Mol Immunol. 1999 Jan;36(1):45-52. doi: 10.1016/s0161-5890(98)00111-4.


Interleukin (IL)-4 plays an important role in IgE synthesis in B cells and in Th2 differentiation in T cells. IL-4 conducts its biological activities through binding to the IL-4 receptor (IL-4R) on the surface of target cells. IL-4R are thought to be composed of the IL-4R alpha chain (IL-4R alpha) and either the IL-2R gamma chain or the IL-13R alpha chain. We have previously shown that the membrane-proximal portion in the cytoplasmic domain of the human IL-4R alpha (hIL-4R alpha) is critical for proliferation, generation of germline epsilon transcript, and activation of STAT6, based on analyses of truncated hIL-4R alphas. In this study, we found that p47phox, an activator of the phagocyte NADPH oxidase, binds to this portion by the two-hybrid system. Furthermore, we observed the association of p47phox with the hIL-4R alpha in B cells derived from a normal donor. These results suggest that p47phox is involved in the signal transduction of IL-4 in B cells. However, activation of STAT6, CD23 expression, and IgE synthesis induced by IL-4 were not affected in p47phox-deficient patients, which raises the possibility that p47phox may be important in other signaling activities as well in B cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • B-Lymphocytes / immunology*
  • Enzyme Activation / drug effects
  • Humans
  • Interleukin-4 / immunology
  • Interleukin-4 / pharmacology*
  • Lymphocyte Activation / drug effects
  • Molecular Sequence Data
  • NADPH Oxidases / immunology
  • NADPH Oxidases / metabolism*
  • Phosphoproteins / immunology
  • Phosphoproteins / metabolism*
  • Receptors, Interleukin-4 / immunology
  • STAT6 Transcription Factor
  • Signal Transduction / immunology
  • Th2 Cells / immunology*
  • Trans-Activators / immunology


  • Phosphoproteins
  • Receptors, Interleukin-4
  • STAT6 Transcription Factor
  • STAT6 protein, human
  • Trans-Activators
  • Interleukin-4
  • NADPH Oxidases
  • neutrophil cytosolic factor 1